Real Madrid player Luka Modric and Marcelo have tested the positive Covid-19, said Laliga Club in a statement on Wednesday.
Real did not say whether they suffered any symptoms, but they had been transferred to quarantine in line with the Spanish health protocol and would miss the league match on Sunday against Cadiz.
The number of Covid-19 cases that grew around Europe also had an impact on the world of football.
Valencia confirmed four cases on Monday, including their head coach and two players.
In the UK, 42 Premier League players and positive tested staff in the past week – the most recording in the league for a seven-day period.
The plague at Tottenham Hotspur led UEFA to postpone their European Conference League matches against France Side Rennes last week.
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