Pune: The Ministry of Health of the Union has suggested the state to adopt the RT-PCR double testing strategy in front of the positive covid sample genome sequencing for omicron detection.
Under the new plan, the alleged samples of throat / nasal nose which are thought to be tested using a standard RT-PCR kit and if it is detected positively, the same sample will undergo the second test with the imported RT-PCR kit has a decrease in s-genes.
“And only samples that show positive for the failure of the target drop-out s-gene or s-gen (SGTF) will be sorted by an omicron.
In addition to cutting costs, it will also help in taking omicron cases that are very possible and save resources, “Senior officials of the Ministry of Health Workers on Monday.
The Maharashtra government is the first to have begun to implement a double test strategy.
Quite a number of districts with high floating populations have received import kits.
“Genome sequencing costs rose to RS 5,000 per positive covid sample in India, including transportation, human resources, and other heads,” the official said, adding that while the cost per kit of the standard RT-PCR kit and imported RT-PCR kit With SGTF each Rs 19 and Rs 240.
Dual RT-PCR costs Rs 260.
In addition to testing individuals with international travel history and their close contact, this kit can also be used in the government managed laboratory for routine Covid tests to override the deployment of the community from High viral loads or below 25 threshold cycles (CT).
A total of 23 of 60 positive covid samples have so far been positively tested for s-gen drop-out in Nagpur.
Instead of sorting all 60 positive samples, 23 this sample was prioritized to sort in Nagpur.
Diagnostic Diagnostic Laboratory Virus in Medical College and Indira Gandhi Nagpur Hospital – One of the main sentinel centers in Maharashtra – was conducting a second RT-PCR test for SGTF before sending samples to sort.
Head of the Microbiology Department of the Hospital and the main investigator of SS Raut said, “Civic and District Administration continues a positive sample of public and private laboratories.
We are testing this sample again using a special RT-PCR kit for SGTF.” The second omicron patient confirmed the two nagpur , a 21-year-old who returned from Dubai, was the first to cover the throat / nasal swab when tested twice with RT-PCR kit showed SGTF.
The sample was then sorted by a genome to confirm the existence of the Omicron variant.
“If someone tests the SGTF positive in the second RT-PCR test, we can take the sample for intact genomic sequencing on priority to confirm.
Opportunities for variant detection of increases in various cases in such cases,” said Dr.
Rajesh Karyakarte, one of the microbiologists The main involved in the Indian Genomics Consortium (Insacog), a 38 network of laboratories to monitor genomical variations in SARS-COV-2.
Countries say to do dual RT-PCR before sequencing
